Repositorio Académico UOH

Bibliotecas Universidad de O'Higgins



Mostrar el registro sencillo del ítem

dc.contributor.author Balboa, E
dc.contributor.author Saavedra, F
dc.contributor.author Cea, LA
dc.contributor.author Ramírez, V
dc.contributor.author Escamilla, R
dc.contributor.author Vargas, AA
dc.contributor.author Regueira, T
dc.contributor.author Sáez, JC
dc.date.accessioned 2024-01-17T15:56:15Z
dc.date.available 2024-01-17T15:56:15Z
dc.date.issued 2020
dc.identifier.uri https://repositorio.uoh.cl/handle/611/971
dc.description.abstract Glucocorticoids are frequently used as anti-inflammatory and immunosuppressive agents. However, high doses and/or prolonged use induce undesired secondary effects such as muscular atrophy. Recently, de novo expression of connexin43 and connexin45 hemichannels (Cx43 HCs and Cx45 HCs, respectively) has been proposed to play a critical role in the mechanism underlying myofiber atrophy induced by dexamethasone (Dex: a synthetic glucocorticoid), but their involvement in specific muscle changes promoted by Dex remains poorly understood. Moreover, treatments that could prevent the undesired effects of glucocorticoids on skeletal muscles remain unknown. In the present work, a 7-day Dex treatment in adult mice was found to induce weight loss and skeletal muscle changes including expression of functional Cx43/Cx45 HCs, elevated atrogin immunoreactivity, atrophy, oxidative stress and mitochondrial dysfunction. All these undesired effects were absent in muscles of mice simultaneously treated with Dex and vitamin E (VitE). Moreover, VitE was found to rapidly inhibit the activity of Cx HCs in freshly isolated myofibers of Dex treated mice. Exposure to alkaline pH induced free radical generation only in HeLa cells expressing Cx43 or Cx45 where Ca2+ was present in the extracellular milieu, response that was prevented by VitE. Besides, VitE and two other anti-oxidant compounds, Tempol and Resveratrol, were found to inhibit Cx43 HCs in HeLa cells transfectants. Thus, we propose that in addition to their intrinsic anti-oxidant potency, some antioxidants could be used to reduce expression and/or opening of Cx HCs and consequently reduce the undesired effect of glucocorticoids on skeletal muscles.
dc.description.sponsorship Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT)(Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT)
dc.description.sponsorship Centro Interdisciplinario de Neurociencias de Valparaiso
dc.relation.uri http://dx.doi.org/10.3390/ijms21114094
dc.subject connexons
dc.subject mitochondrial dysfunction
dc.subject oxidative stress
dc.subject muscle atrophy
dc.subject dexamethasone
dc.title Vitamin E Blocks Connexin Hemichannels and Prevents Deleterious Effects of Glucocorticoid Treatment on Skeletal Muscles
dc.type Artículo
uoh.revista INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
dc.identifier.doi 10.3390/ijms21114094
dc.citation.volume 21
dc.citation.issue 11
dc.identifier.orcid Saavedra, Fujiko/0000-0002-1369-6022
dc.identifier.orcid Saez, Juan/0000-0003-3811-0347
dc.identifier.orcid Escamilla Hernandez, Rosalba/0000-0002-4491-3907
uoh.indizacion Web of Science


Ficheros en el ítem

Ficheros Tamaño Formato Ver

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem


Colecciones


Archivos

Artículos

Tesis

Videos


Cuartiles