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dc.contributor.author González-Candia, A
dc.contributor.author Candia, AA
dc.contributor.author Paz, A
dc.contributor.author Mobarec, F
dc.contributor.author Urbina-Varela, R
dc.contributor.author del Campo, A
dc.contributor.author Herrera, EA
dc.contributor.author Castillo, RL
dc.date.accessioned 2024-01-17T15:54:07Z
dc.date.available 2024-01-17T15:54:07Z
dc.date.issued 2022
dc.identifier.uri https://repositorio.uoh.cl/handle/611/364
dc.description.abstract More than 80 million people live and work (in a chronic or intermittent form) above 2500 masl, and 35 million live in the Andean Mountains. Furthermore, in Chile, it is estimated that 100,000 people work in high-altitude shifts, where stays in the lowlands are interspersed with working visits in the highlands. Acute exposure to high altitude has been shown to induce oxidative stress in healthy human lowlanders due to increased free radical formation and decreased antioxidant capacity. However, intermittent hypoxia (IH) induces preconditioning in animal models, generating cardioprotection. Here, we aim to describe the responses of a cardiac function to four cycles of intermittent hypobaric hypoxia (IHH) in a rat model. The twelve adult Wistar rats were randomly divided into two equal groups, a four-cycle of IHH and a normobaric hypoxic control. Intermittent hypoxia was induced in a hypobaric chamber in four continuous cycles (1 cycle = 4 days of hypoxia + 4 days of normoxia), reaching a barometric pressure equivalent to 4600 m of altitude (428 Torr). At the end of the fourth cycle, cardiac structural and functional variables were also determined by echocardiography; furthermore, cardiac oxidative stress biomarkers (4-Hydroxynonenal, HNE; nitrotyrosine, NT), antioxidant enzymes, and NLRP3 inflammasome panel expression are also determined. Our results show a higher ejection and a shortening fraction of the left ventricle function by the end of the fourth cycle. Furthermore, cardiac tissue presented a decreased expression of antioxidant proteins. However, a decrease in IL-1 beta, TNF-alpha n, and oxidative stress markers is observed in IHH compared to normobaric hypoxic controls. Non-significant differences were found in protein levels of NLRP3 and caspase-1. IHH exposure determines structural and functional heart changes. These findings suggest that initial states of IHH are beneficial for cardiovascular function and protection.
dc.description.sponsorship Santander Universia
dc.description.sponsorship Proyecto Puente-ICBM 2019
dc.description.sponsorship Fondecyt(Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT)
dc.description.sponsorship Fondecyt Iniciacion(Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT)
dc.description.sponsorship CONICYT PFCHA/DOCTORADO NACIONAL
dc.relation.uri http://dx.doi.org/10.3390/antiox11061043
dc.subject intermittent hypoxia
dc.subject NLRP3 inflammasome
dc.subject antioxidant defenses
dc.subject NF-kappaB
dc.subject cardioprotection
dc.title Cardioprotective Antioxidant and Anti-Inflammatory Mechanisms Induced by Intermittent Hypobaric Hypoxia
dc.type Artículo
uoh.revista ANTIOXIDANTS
dc.identifier.doi 10.3390/antiox11061043
dc.citation.volume 11
dc.citation.issue 6
dc.identifier.orcid Gonzalez-Candia, Alejandro/0000-0001-8429-367X
dc.identifier.orcid del Campo, Andrea/0000-0003-3830-7334
dc.identifier.orcid Paz, Adolfo A./0000-0003-4611-2748
dc.identifier.orcid Herrera, Emilio A/0000-0002-6342-085X
uoh.indizacion Web of Science


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